# Sort and Layout¶

**Author**: Simon Heumos

## Synopsis¶

Pangenome graphs built from raw sets of alignments may have complex structures which can introduce difficulty in downstream analyses, visualization, mapping, and interpretation. Graph sorting aims to find the best node order for a 1D and 2D layout to simplify these complex regions. This tutorial shows how to sort and visualize a graph in 1D. It explains how to generate a 2D layout of a graph, and how to take a look at the calculated layout using static and interactive tools.

Note

Be aware that odgi sort offers much more sorting algorithms than this tutorial could cover here in detail.

## 1D sorting¶

### Build the unsorted DRB1-3123 graph¶

Assuming that your current working directory is the root of the `odgi`

project, to construct an `odgi`

graph from the
`DRB1-3123`

dataset in `GFA`

format, execute:

```
odgi build -g test/DRB1-3123_unsorted.gfa -o DRB1-3123_unsorted.og
```

The command creates a file called `DRB1-3123_unsorted.og`

, which contains the input graph in `odgi`

format. This graph contains
12 ALT sequences of the HLA-DRB1 gene from the GRCh38 reference genome.
The graph was initially created with the seqwish variation graph inducer which produces unsorted, raw graphs from
all versus all alignments of the input sequences.

### Visualize the unsorted DRB1-3123 graph¶

```
odgi viz -i DRB1-3123_unsorted.og -o DRB1-3123_unsorted.png
```

To obtain the following PNG image:

In this 1-Dimensional visualization:

The graph nodes are arranged from left to right, forming the

`pangenome sequence`

.The colored bars represent the binned, linearized renderings of the embedded paths versus this

`pangenome sequence`

in a binary matrix.The path names are visualized on the left.

The black lines under the paths are the links, which represent the graph topology.

The graph is very complex and, in this form, the underlying structure is unclear, making it difficult to reason from it. odgi offers algorithms to find the best 1D node order effectively linearizing the graph.

### 1D layout metrics of the unsorted DRB1-3123 graph¶

odgi stats provides metrics to evaluate the goodness of the sort of a variation graph. Let's take a look:

```
odgi stats -i DRB1-3123_unsorted.og -s -d -l -g
```

Where:

`-s`

calculates the sum of path node distances.`-l`

calculates the mean links length.`-d`

additionally penalizes links which connect nodes with different orientation.`-g`

ensures that gap links, links directly travelling from left to right encoding simple structural variants, are not penalized.

For further details on these metrics please take a look at the odgi stats command.

We observe on stdout:

```
#mean_links_length
path in_node_space in_nucleotide_space num_links_considered num_gap_links_not_penalized
all_paths 514.698 4016.92 21870 11116
#sum_of_path_node_distances
path in_node_space in_nucleotide_space nodes nucleotides num_penalties num_penalties_different_orientation
all_paths 1029.84 1076.32 21882 163416 6085 1
```

### Sort the unsorted DRB1-3123 graph in 1D¶

Let's sort the graph:

```
odgi sort -i DRB1-3123_unsorted.og --threads 2 -P -Y -o DRB1-3123_sorted.og
```

`-Y`

selects the PG-SGD algorithm for sorting. This algorithm moves a single pair of nodes at a time, optimizing
the disparity between the layout distance of a node pair and the actual nucleotide distance of a path traversing these
nodes.

Note

The PG-SGD is not deterministic, because of its Hogwild! approach.

### Visualize the 1D sorted DRB1-3123 graph¶

```
odgi viz -i DRB1-3123_sorted.og -o DRB1-3123_sorted.png
```

The graph lost it's complexity and is now linear.

**Bonus**: the following animation represents what happens during the PG-SGD sorting.

### 1D layout metrics of the sorted DRB1-3123 graph¶

```
odgi stats -i DRB1-3123_sorted.og -s -d -l -g
```

This prints to stdout:

```
#mean_links_length
path in_node_space in_nucleotide_space num_links_considered num_gap_links_not_penalized
all_paths 2.15542 15.0529 21870 9481
#sum_of_path_node_distances
path in_node_space in_nucleotide_space nodes nucleotides num_penalties num_penalties_different_orientation
all_paths 4.66114 4.72171 21882 163416 5948 1
```

Compared to before, these metrics show that the goodness of the sorting of the graph improved significantly.

## 1D reference-guided grooming and reference-guided sorting¶

In the following, we will groom and sort a human MHC pangenome graph with respect to given references.

### Extract the MHC graph from Chr6¶

```
prefix_chr6_smooth=chr6.pan.fa.a2fb268.4030258.6a1ecc2.smooth
# download and build the graph
wget https://s3-us-west-2.amazonaws.com/human-pangenomics/pangenomes/scratch/2021_11_16_pggb_wgg.88/chroms/${prefix_chr6_smooth}.gfa.gz
gunzip ${prefix_chr6_smooth}.gfa.gz
odgi build -g ${prefix_chr6_smooth}.gfa -o ${prefix_chr6_smooth}.og -t 16 -P
# extraction and optimization of the MHC locus
odgi extract -i ${prefix_chr6_smooth}.og -r grch38#chr6:29000000-34000000 -o - -c 0 --full-range -t 16 -P | odgi sort -i - -o ${prefix_chr6_smooth}.mhc.og --optimize
```

### Visualize the MHC by orientation¶

```
odgi viz -i${prefix_chr6_smooth}.mhc.og -o ${prefix_chr6_smooth}.mhc.og.z.png -z
```

Overview of the node orientation of the MHC graph. How would the graph would like if we want to ensure that path `HG00733#2#JAHEPP010000166.1:0-551833`

is in forward orientation?

### Groom the MHC by haplotype HG00733#2¶

```
# collect our desired path in a file
odgi paths -i ${prefix_chr6_smooth}.mhc.og -L | head -n 12 | tail -n 1 > HG00733_1
# apply reference-guided grooming
odgi groom -i ${prefix_chr6_smooth}.mhc.og -R HG00733_1 -o ${prefix_chr6_smooth}.mhc.og.groom.og
# take another look at the orientation of path ``HG00733#2#JAHEPP010000166.1:0-551833``
odgi viz -i${prefix_chr6_smooth}.mhc.og.groom.og -o ${prefix_chr6_smooth}.mhc.og.groom.z.png -z
```

The selected graph is now forward oriented. But what if we want to ensure that all nodes touching our precious references are in forward orientation?

### Groom the MHC by haplotypes CHM13 and GRCh38¶

```
# collect our desired path in a file
odgi paths -i ${prefix_chr6_smooth}.mhc.og -L | head -n 2 > CHM13_GRCh38
# apply reference-guided grooming
odgi groom -i ${prefix_chr6_smooth}.mhc.og -R CHM13_GRCh38 -o ${prefix_chr6_smooth}.mhc.og.groom_chm13_grch38.og
# take another look at the orientation of the reference paths
odgi viz -i ${prefix_chr6_smooth}.mhc.og.groom_chm13_grch38.og -o ${prefix_chr6_smooth}.mhc.og.groom_chm13_grch38.og.z.png -z
```

Now the two reference paths are in forward orientation again, as before :)

### Visualize the MHC by path position¶

```
odgi viz -i${prefix_chr6_smooth}.mhc.og -o ${prefix_chr6_smooth}.mhc.og.du.png -du
```

Assuming we now want to force the 1D layout to respect the path positions of the references better, how to do that?

### Sort the MHC by paths CHM13 and GRCh38¶

```
# apply reference-path-guided stochastic gradient descent - rPG-SGD
odgi sort -i ${prefix_chr6_smooth}.mhc.og -H CHM13_GRCh38 -o ${prefix_chr6_smooth}.mhc.og.Y_chm13_grch38.og -t 16 -P -Y
# take another look at the orientation of the reference paths
odgi viz -i ${prefix_chr6_smooth}.mhc.og.Y_chm13_grch38.og -o ${prefix_chr6_smooth}.mhc.og.Y_chm13_grch38.og.du.png -du
```

We can clearly observe, that the path positions of the two reference now define the graph. Both reference show greater continuity compared to before.

## 2D layout¶

### 2D layout of the unsorted DRB1-3123 graph¶

We want to have a 2D layout of our DRB1-3123 graph:

```
odgi layout -i DRB1-3123_unsorted.og -o DRB1-3123_unsorted.og.lay -P --threads 2
```

### Drawing the 2D layout of the DRB1-3123 graph¶

Calculate the 2D layout:

```
odgi draw -i DRB1-3123_unsorted.og -c DRB1-3123_unsorted.og.lay -p DRB1-3123_unsorted.og.lay.png -C -w 50
```

**Bonus**: the following animation represents what happens during the layout generation.

### Interactive 2D visualization with gfaestus¶

gfaestus is a Vulkan-accelerated 2D GFAv1 interactive visualization tool. It currently requires a GFA and an accompanied layout file in TSV format from odgi layout.

We can create the TSV with another run of odgi draw:

```
odgi draw -i DRB1-3123_unsorted.og -c DRB1-3123_unsorted.og.lay -p DRB1-3123_unsorted.og.lay.png -H 500 -C -w 10 -T DRB1-3123_unsorted.og.lay.tsv
```

Or, if the layout was not created, yet, we can run odgi layout:

```
odgi layout -i DRB1-3123_unsorted.og -o DRB1-3123_unsorted.og.lay -P --threads 2 -T DRB1-3123_unsorted.og.tsv
```

Now download the source code of `gfaestus`

and compile the Rust code:

```
git clone --recursive https://github.com/chfi/gfaestus.git
cargo build --release
```

Assuming you made the resulting binary in `target/release/gfaestus`

globally available, you can run:

```
gfaestus test/DRB1-3123_unsorted.gfa DRB1-3123_unsorted.og.tsv
```

Then `gfaestus`

will show up: